Evaluation of Candida SPP involvement in women ingesting contraceptives according to the latest classification
DOI:
https://doi.org/10.56294/hl2024.538Keywords:
Candida albicans, oral contraceptives, periodontal microbiota, periodontal disease, biofilmsAbstract
Introduction: The study evaluated the relationship between contraceptive use and the presence of *Candida* species in the periodontal microbiome of women. *Candida albicans* was identified as the most common species, although others such as *C. parapsilosis* and *C. tropicalis* were also detected. These yeasts showed a higher prevalence in inflammatory conditions and in advanced stages of periodontal disease. In addition, the influence of female hormones and contraceptives in the alteration of the periodontal microenvironment, favoring the growth of opportunistic species, was considered.
Methods: A cross-sectional study was carried out in 100 women, divided into two groups: contraceptive users (n=50) and non-users (n=50). Microbiological samples were collected from various oral surfaces using culture techniques, chromogenic methods and PCR. Participants included immunocompetent women with various periodontal conditions, excluding those with systemic diseases, recent antimicrobial use or smoking habits.
Results: Eighty-two *Candida* isolates were identified, with *C. albicans* (34) and *C. parapsilosis* (16) standing out. There were no significant differences in *Candida* colonization between healthy women and women with gingivitis, regardless of contraceptive use. However, in advanced stages of periodontitis, the prevalence of *Candida* was significantly increased in contraceptive users.
Conclusions: Contraceptive use did not significantly influence *Candida* colonization in healthy or gingivitis women, but it did in advanced stages of periodontitis. These findings highlight the importance of monitoring periodontal microbiota in women with hormonal changes or hormonal treatments, considering the potential impact of *Candida* as a reservoir of opportunistic infections.
References
Ademe M, Girma F. Candida auris: From multidrug resistance to pan-resistant strains. Infect Drug Resist. 2020;13:1287–94.
Avila M, Ojcius DM, Yilmaz O. The oral microbiota: living with a permanent guest. DNA Cell Biol. 2009;28(8):405–11.
Benito B, Aranda S, López F, de la Rosa E, Rosas R, Sánchez L. Oral Candida isolates and fluconazole susceptibility patterns in older Mexican women. Arch Gerontol Geriatr. 2016;65:204–10.
Borgo P, Rodrigues V, Feitosa A, Xavier K, Avila-Campos M. Association between periodontal condition and subgingival microbiota in women during pregnancy: a longitudinal study. J Appl Oral Sci. 2014;22(6):528–33.
Brusca M, Verdugo F, Amighini C, Albaina O, Moragues M. Anabolic steroids affect human periodontal health and microbiota. Clin Oral Investig. 2014;18(6):1579–86.
Brusca M, Rosa A, Olatz A, Moragues M, Verdugo F, Pontón J. The impact of oral contraceptives on women's periodontal health and the subgingival occurrence of aggressive periodontopathogens and Candida species. J Periodontol. 2010;81(7):1010–8.
Bulacio L, Paz M, Ramadan S, et al. Oral infections caused by yeasts in patients with head and neck cancer undergoing radiotherapy: Identification of the yeasts and evaluation of their antifungal susceptibility. J Mycol Med. 2012;22(4):348–53.
Carrillo de Albornoz A, Figuero E, Herrera D, Bascones A. Gingival changes during pregnancy: I. Influence of hormonal variations on clinical and immunological parameters. J Clin Periodontol. 2010;37(3):220–9.
Corgel J. Dental management of the female patient. J Clin Periodontol 2000. 2013;61:219–31.
Cuya-García R, Chávez-Raymi A, Flores-Culqui S, Párraga-Navarro M, Quinto-Benalcázar R, Tafur-Vásquez O. Enfermedad periodontal asociada al embarazo. Rev Cient Odontol (Lima). 2019;7(1):132–9.
De La Torre J, Quindos G, Marcos-Arias C, Marichalar-Mendia X, Gainza ML, Eraso E, et al. Oral Candida colonization in patients with chronic periodontitis: Is there any relationship? Rev Iberoam Micol. 2018;35:134–9.
GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990–2016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017;390(10100):1211–59.
Jain M, Shah R, Chandolia B, et al. The oral carriage of Candida in oral cancer patients of Indian origin undergoing radiotherapy and/or chemotherapy. J Clin Diagn Res. 2016;10(2):17–20.
Javed F, Al-Kheraif A, Kellesarian S, Vohra F, Romanos G. Oral Candida carriage and species prevalence in denture stomatitis patients with and without diabetes. J Biol Regul Homeost Agents. 2017;31(2):343–6.
Jenkinson HF, Lamont RJ. Oral microbial communities in sickness and in health. Trends Microbiol. 2005;13(12):589–95.
Jewtuchowicz VM, Mujica MT, Malzone MC, Cuesta A, Brusca MI, Nastri ML, et al. Origin of the colonization of Candida dubliniensis in subgingival biofilm in immunocompetent subjects by RAPD-PCR assay. J Oral Microbiol. 2009;1(1):507.
Jewtuchowicz VM, Mujica MT, Brusca MI, Sordelli N, Malzone MC, Pola SJ, et al. Phenotypic and genotypic identification of Candida dubliniensis from subgingival sites in immunocompetent subjects in Argentina. Oral Microbiol Immunol. 2008;23(6):505–9.
Jewtuchowicz VM, Brusca MI, Mujica MT, Gliosca LA, Finquelievich JL, Iovannitti CA, Rosa AC. Subgingival distribution of yeast and their antifungal susceptibility in immunocompetent subjects with and without dental devices. Acta Odontol Latinoam. 2007;20:17–22.
Melton J, Redding S, Kirkpatrick W, et al. Recovery of Candida dubliniensis and other Candida species from the oral cavity of subjects with periodontitis who had poorly controlled type 2 diabetes: A pilot study. Spec Care Dentist. 2010;30(6):230–4.
Muthular M, Bálsamo F, Passero P, Jewtuchowicz V, Miozza V, Villalba MB, et al. Effects of tamoxifen on periodontal disease and Candida albicans of patients with breast cancer and other pathologies. Future Microbiol. 2019;14:129–37.
Newman MG, Takei HH, Klokkevold PR, Carranza FA. Newman and Carranza’s Clinical Periodontology. 13th ed. Philadelphia: Elsevier Health Sciences; 2019.
Mitova N, Rashkova M. Sex hormones in the saliva and periodontal health of children in puberty. J IMAB. 2019;25(4):2874–8.
Pazmiño VFC, Assem NZ, Pellizzer EP, De Almeida JM, Theodoro LH. Influencia del estrógeno en la enfermedad periodontal: Revisión de literatura. Av Periodon Implantol. 2015;27(2):75–8.
Preshaw P. Oral contraceptives and the periodontium. J Clin Periodontol 2000. 2013;61:125–59.
Rodríguez L, Rosa A, Rodríguez J, et al. The oral cavity: A reservoir that favors colonization and selection of Candida parapsilosis sensu stricto strains with high pathogen potential under conditions of gingival-periodontal disease. J Dent Sci Ther. 2017;2(1):1–6.
Rodríguez L, Jewtuchowicz V. Molecular characterization of Candida parapsilosis species complex in niches of the oral cavity in a cohort of patients from Argentina with different oral and dental clinical manifestations. J Dent Sci Ther. 2016;1(1):18–25.
Seneviratne CJ, Jin L, Samaranayake LP. Biofilm lifestyle of Candida: A mini review. Oral Dis. 2008;14(6):582–90.
Silk H. Diseases of the mouth. Prim Care. 2014;41(1):75–90.
Terças A, Marques S, Monteiro C, et al. Antifungal drug susceptibility of Candida species isolated from HIV-positive patients recruited at a public hospital in São Luis, Maranhão, Brazil. Front Microbiol. 2017;8:298.
Theill L, Dudiuk C, Morales-Lopez S, Berrio I, Rodríguez JY, Marin A, et al. Single-tube classical PCR for Candida auris and Candida haemulonii identification. Rev Iberoam Micol. 2018;35(2):110–2.
Truong T, Pang LM, Rajan S, Wong SSW, Fung YME, Samaranayake L, et al. The proteome of community living Candida albicans is differentially modulated by the morphologic and structural features of the bacterial cohabitants. Microorganisms. 2020;8(10):1541.
Yap M, Alnuaimi A, Adams G, McCullough M. Oral candidal carriage in asymptomatic patients. Aust Dent J. 2016;61(2):190–5.
Herrera D, Figuero E, Shapira L, Jin L, Sanz M. La nueva clasificación de enfermedades periodontales y periimplantarias. Rev Cient Soc Esp Periodoncia. 2018;11:1–8.
Looney WJ, Muhlemann K, Narita M. Stenotrophomonas maltophilia: An emerging opportunistic human pathogen. Lancet Infect Dis. 2009;9(5):312–23.
Jun L, Ying L, Yu Z, Changzheng W, Benyan W, Jun W. Actinomyces and alimentary tract diseases: A review of its biological functions and pathology. Biomed Res Int. 2018;2018:3820215.
Santarelli A, Mascitti M, Galeazzi R, Marziali A, Busco F, Procaccini M. Oral ulcer by Sphingomonas paucimobilis: First report. Int J Oral Maxillofac Surg. 2016;45(3):1–5.
Handschuh-Briones RA, Silva-Arcos EN, Urrutia M, Godoy-Martínez P. Actividad antifúngica de los enjuagues bucales frente a Candida albicans y Rhodotorula mucilaginosa: Un estudio in vitro. Rev Iberoam Micol. 2020;37(2):78–83.
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Copyright (c) 2024 Edward Alejandro Hansen, María Isabel Brusca, María Laura Garzon, Atilio Vela Ferreira, Virginia Jewtuchowicz (Author)
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