Comparison of healing activity between azadirachta indica and moringa oleifera seed oils on cutaneous wounds in wistar rats

Abstract

Medicinal plants are natural remedies that bring benefits to the organism. Phytomedicine’s production has increased in the last decades due to the adverse reactions of designer drugs. This research focused on basic principles that rule the scarification of wounds as a natural response to the damages produced with specific functions of tissue restoration and integrity. For this reason the acceleration of the scarring effect with Azadirachta indica seeds oil and Moringa was studied. Nine groups of animals were made: normal, negative and negative control, three Neem groups, three Moringa groups (30, 60.120 µl) respectively. The wound was made after placing the animal under anesthesia using the punch method. For seven days, these groups were observed while they received the treatment. After the test, the animals were euthanized, and scarred skin tissue samples were collected for histopathologic tests. As a result, after the second day, the concentration of Neem tested 4,38 mg/ml showed humity and inflammation of 73% and 76% respectively, which eventually dropped to zero. The presence of crust in the third day was 20% and in the sixth, 100%. On the other hand, concerning to the Moringa oil 4,48 mg/ml, no significant changes could be seen in any of the evaluated parameters. Comparing the percentages of the crust coverage among these groups, it can be said that: natural scarification
(B) 10% the third day and 40% at the end of the research. The subjects treated with neem (E) 30% the third day and 100% the sixth, while D, F 80% the same day and 100% the seventh, the same as seen in C (iodine). The groups with Moringa presented 10% in the third day and 60% by the end. In conclusion, the groups with neem showed acceleration in the scarification process within the experimentation period.