Cytoprotective effect of allium sativum (garlic) and alkaline water on β-pancreatic cells as a preventive treatment for diabetes induced in wistar rats

Abstract

Allium sativum and alkaline water have an antioxidant effect, capable of protecting any cell from degeneration. Studies have shown that they regulate glucose levels; however, there is no evidence of research on the mixture of both treatments with histopathological studies. The objective of the present study was to evaluate the cytoprotective effect of the joint administration of Allium sativum and Alkaline Water in different doses and periods of time. For this research, 8 groups of 12 animals each were formed, to which initial glucose was measured, and then the corresponding treatments were administered for 7, 14 and 21 days. Control group A without treatment, group B induction with alloxan, group C positive control administered: Glibenclamide, Group D Allium sativum oil, Group E alkaline water and Groups F, G, and H received Allium sativum and Alkaline water together at different doses. After each time period diabetes was induced using alloxan, final glucose was measured and the pancreas was extracted for subsequent histopathological analysis.
The results obtained were analyzed by comparison of means, observing that from 7 days of treatment, both Allium sativum and alkaline water separately, as well as the joint administration of both, prevented the increase of glucose values. Histopathological analysis showed that Allium sativum had a cytoprotective effect at 21 days of treatment; on the other hand, at 21 days of treatment with alkaline water, a minimal cytoprotective effect was observed; while experimental animals treated with both treatments at different doses showed a cytoprotective effect from day 7 of treatment. Concluding that the joint administration of Allium sativum and alkaline water has an effective cytoprotective and normoglycemic effect.